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KMID : 1150720210100020012
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Integrative Medicine Research
2021 Volume.10 No. 2 p.12 ~ p.12
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The effect of acupuncture stimulation on alleviating emotional changes due to acute alcohol administration and the possibility of sigma1 receptor involvement
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Seo Su-Yeon
Kim Soo-Phil
Bang Se-Kyun
Kang Suk-Yun
Cho Seong-Jin
Choi Kwang-Ho
Ryu Yeon-Hee
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Abstract
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Background: Most ETOH addiction preclinical studies have focused on the rewards of chronic ETOH self-administration or the ETOH reinstatement model. Acute ETOH administration studies are scarce despite the potential of ETOH to cause sedation, intoxication and reduced acute functional tolerance. Here, we established a rat model of acute ETOH administration induced by an intraperitoneal injection of 1?g/kg ethanol and assessed the similarities in physiological and behavioral effects between acupuncture and Sigma1 R antagonists.
Methods: Male Wistar rats (300?330?g) received pretreatment with (1) saline injection, (2) saline?+?mechanical stimulation using a mechanical acupuncture instrument (MAI) for acupuncture at the Shenmen (HT7), (3) ETOH (1?g/kg) injection, (4) ETOH?+?HT7, or (5) the selective σ1 R antagonist BD 1047 (3, 10, or 30?mg/kg, intraperitoneal (IP) injection). ETOH (1?g/kg) or saline was IP injected after 10?min. Then, ETOH-induced immobility was evaluated in an open field arena, ultrasonic vocalizations (USVs) indicating ethanol-induced emotional changes were recorded in a recording chamber, and the rats were sacrificed for the analysis of protein levels of σ1 R in several regions of the brain.
Results: Acute ethanol exposure increased the immobile time, 22-kHz USVs, and protein levels of σ1 R in the ventral tegmental area (VTA). However, pretreatment with acupuncture at HT7 induced recovery of immobile time, reduced 22-kHz USVs, and regulated the protein levels of σ1 R in the VTA. These effects have similarities with IP injection of BD 1047 (10?mg/kg).
Conclusion: This study showed that acupuncture at HT7 regulates immobility and 22-kHz USVs via Sigma1 R in the VTA upon acute ETOH exposure.
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KEYWORD
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Acupuncture, Acute ethanol, Sigma-1 receptor, Ultrasonic vocalizations, VTA
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